ENZYME HISTOCHEMISTRY

Enzyme histochemistry of skeletal muscle.

This is the first of three papers on the application of enzyme histochemical techniques to the study of developing and diseased muscle. This paper will deal with developing muscle in various laboratory animals, the second with developing human muscle, and the third with hereditary neurogenic atrophies in infancy and childhood. In 1678 Stefano Lorenzini had already observed that animal muscle co...

Enzyme histochemistry of acute staphylococcal enterotoxin gastroenteritis in rhesus monkeys.

Current concepts of enzyme histochemistry in modern pathology.

Enzyme histochemistry serves as a link between biochemistry and morphology. It is based on metabolization of a substrate provided to a tissue enzyme in its orthotopic localization. Visualization is accomplished with an insoluble dye product. It is a sensitive dynamic technique that mirrors even early metabolic imbalance of a pathological tissue lesion, combined with the advantage of histotopogr...

Enzyme histochemistry on jejunal tissue embedded in resin.

Plastination of Whole Animal Preparations following Histochemistry: in Situ Localization of the Enzyme Acetylcholinesterase

A method for whole animal acetylcholinesterase (AChE) histochemistry was combined with standard S10 plastination. Resultant whole or partial animal specimens prepared in this manner were superior to whole animal specimens processed for AChE histochemistry and stored in formalin. The results compared favorably with those attained with isolated organ or tissue whole mounts while preserving and en...