Enzyme histochemical analysis on cryostat sections of human bone marrow.

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Enzyme histochemical analysis on cryostat sections of human bone marrow.

A recently developed procedure, that has been shown to be suitable for detailed immunohistological analysis, has been used to prepare cryostat sections of bone marrow to investigate whether enzyme-histochemical techniques are also feasible on such material. A selected group of enzymes, some of which are inhibited or destroyed in paraffin- or plastic-embedded samples, have been demonstrated. The...

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Reticuloendothelial cells (RES cells) of human bone marrow were enzyme histochemically and cytochemically studied. Acid phosphatase staining (azo-dye method) was most suitable for the demonstration of RES cells. Attempts were made to classify RES cells of smear specimens into 5 types according to the size of nucleus, volume of cytoplasm and phagocytosis with the aid of acid phosphatase staining...

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Preparation of unfixed undemineralized bone sections: the Bright bone cryostat.

A bone cryostat is described with which cold sections of unfixed, undemineralized calcified tissue can be prepared. The bone cryostat is suitable for the preparation of the sections required for the histochemical and immunofluorescent study of bone, for electron microprobe analysis, and for bone and joint autoradiography with soluble compounds.

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I. Histochemical Staining of Bone Marrow Smears

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1982

ISSN: 0021-9746

DOI: 10.1136/jcp.35.11.1220