Enzymatic response of ryegrass cellulose and hemicellulose valorization introduced by sequential alkaline extractions

Abstract Background In view of the natural resistance hemicelluloses in lignocellulosic biomass on bioconversion cellulose into fermentable sugars, alkali extraction is considered as an effective method for gradually fractionating and increasing efficiency cellulose. present study, sequential alkaline extractions were performed delignified ryegrass material to achieve high comprehensively investigated structural features hemicellulosic fractions further applications. Results Sequential removed from cellulose-rich substrates degraded part amorphous cellulose, reducing yields 73.0 27.7% crystallinity indexes 31.7 41.0%. Alkaline enhanced by removal swelling enzymatic hydrolysis 72.3 95.3%. addition, fractionated six fractions, containing arabinoxylans main polysaccharides β -glucans. Simultaneously, concentration polysaccharides, which resulted a decreasing their molecular weights 67,510 50,720 g/mol. Conclusions The study demonstrated that conditions had significant effects investigation physicochemical properties hemicellulose. Overall, will provide useful information efficient utilization biorefineries.