Enzymatic methylation of carboxyl groups of chromaffin granule membrane proteins.
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چکیده
منابع مشابه
Tensile strength of the chromaffin granule membrane.
Catecholamine release from chromaffin granules, suspended in sucrose solutions of various osmotic strengths, was determined at different temperatures between 2 degrees and 44 degrees C. Dynamic measurements showed that steady state is achieved within 15 min of incubation at all temperatures. The effect of temperature on the release was established in terms of the median granular fragility (MGF)...
متن کاملPassive ion permeability of the chromaffin-granule membrane.
'Ghosts' of bovine chromaffin granules, in which the complex mixture of proteins and solutes normally found in the granule matrix is replaced by buffered sucrose are osmotically sensitive. They shrink when the osmotic pressure of the suspension medium is increased, and swell if solute entry is facilitated by the addition of ionophores. Swelling in the presence of ionophores has been used to inv...
متن کاملTransport of Ca2+ and Na+ across the chromaffin-granule membrane.
Bovine chromaffin-granule ghosts accumulate 45Ca2+ in a temperature- and osmotic-shock-sensitive process; the uptake is saturable, with Km 38 microM and Vmax. 28 nmol/min per mg at 37 degrees C. Entry occurs by exchange with Ca2+ bound to the inner surface of the membrane. It is inhibited non-competitively by Na+, La3+ and Ruthenium Red (Ki 10.7 mM, 7 microM and 2 microM respectively), and comp...
متن کاملPhosphatidylinositol kinase. A component of the chromaffin-granule membrane.
Phosphorylation of bovine chromaffin granules by ATP leads to the formation of diphosphoinositide in the granule membrane. Both phosphatidylinositol kinase and its substrate are components of this membrane, and triphosphoinositide is not formed under the conditions of the assay. The reaction is Mg(2+)-dependent and is stimulated by Mn(2+) and F(-) ions. The initial reaction is rapid, with a bro...
متن کاملHydroxytryptamine transport by the bovine chromaffin-granule membrane.
5-Hydroxytryptamine is accumulated by resealed chromaffin-granule 'ghosts' if a pH gradient (acid inside) is imposed across their membranes by preincubating them at low pH. This uptake, like that driven by MgATP, is reserpine- and uncoupler-sensitive. This strongly suggests that catecholamines are taken up by intact granules in response to a pH gradient. In line with this, it is shown that 5-hy...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1978
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)34755-5