Enzymatic Friedel?Crafts Alkylation Using Squalene?Hopene Cyclases

The enzymatic synthesis of fatty acid methyl esters by carboxyl group alkylation.

The reaction was studied by incubation of fatty acid with S-adenosyhnethionine-methylJ4C and isolation of the labeled ester. This was characterized by thin layer and gas-liquid chromatography and by isolation of labeled methanol from alkaline hydrolysates of the ester. Of several substrates tested only S-adenosylmethionine was an effective methyl donor (K,,, = 2.5 X 10F5 M) and oleic acid was t...

Enzymatic Synthesis of Methionine. Chemical Alkylation of the Enzyme-bound Cobamide.

Light-induced free radical alkylation of polynucleotides and their enzymatic digestion.

Ultraviolet light-induced free radical alkylation with 2-propanol or D-ribose, initiated with di-tert-butyl peroxide, of poly (G), poly (U20G), and poly(A) led to the substitution of the appropriate group for the H-8 atom of the purines and addition across the 5,6-double bond of the pyrimidines. The alkylated polynucleotides were subjected to nucleolytic digestion with several nucleases. T1-RNa...

Enzymatic Analysis of Iranian Echis carinatus Venom Using Zymography

Snakebite is a common problem especially in tropical areas all over the world includingIran. Echis carinatus as one of the most dangerous Iranian snakes is spreading in this countryexcluding central and northwest provinces. In this study gelatinase and fibrinogenolyticproperties as two disintegrating matrix metalloproteinase enzymes were evaluated by a strongclear halo between 56-72 kDa in addi...

Enzymatic Analysis of Iranian Echis carinatus Venom Using Zymography

Snakebite is a common problem especially in tropical areas all over the world includingIran. Echis carinatus as one of the most dangerous Iranian snakes is spreading in this countryexcluding central and northwest provinces. In this study gelatinase and fibrinogenolyticproperties as two disintegrating matrix metalloproteinase enzymes were evaluated by a strongclear halo between 56-72 kDa in addi...