Enumeration of Megasphaera elsdenii in rumen contents by real-time Taq nuclease assay
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چکیده
منابع مشابه
Detection and enumeration of Cryptosporidium oocysts in environmental water samples by Real-time PCR assay
Introduction: The protozoan parasite, Cryptosporidium Spp., widely spreads in both raw and drinking waters. It is the causative agents of waterborne diarrhea and gastroenteritis in the world. In the present study, a molecular assay was used for the detection and quantification of Cryptosporidium oocysts in environmental water samples. Materials and methods: Thirty surface water samples wer...
متن کاملMegasphaera elsdenii endocarditis.
A case of endocarditis caused by Megasphaera elsdenii is reported. This anaerobic grim-negative coccus has rarely been associated with human infections and has not previously been described as a cause of endocarditis.
متن کاملButyryl-CoA dehydrogenase from Megasphaera elsdenii
The absorption coefficient of butyryl-CoA dehydrogenase from Megasphaera elsdenii at 450nm is determined as 14.4mM-1 cm-1 in the CoA-free form and 14.2mM-1 cm-1 in the CoA-liganded form (both yellow). The latter value is considerably higher than the earlier published estimate. Phenazine ethosulphate offers great advantages over phenazine methosulphate as a coupling dye in the catalytic assay de...
متن کاملdetection and enumeration of cryptosporidium oocysts in environmental water samples by real-time pcr assay
introduction: the protozoan parasite, cryptosporidium spp., widely spreads in both raw and drinking waters. it is the causative agents of waterborne diarrhea and gastroenteritis in the world. in the present study, a molecular assay was used for the detection and quantification of cryptosporidium oocysts in environmental water samples. materials and methods: thirty surface water samples were col...
متن کاملCatabolism of Amino Acids by Megasphaera elsdenii LC1.
The amino acids in an acid hydrolysate of casein were catabolized more extensively by Megasphaera elsdenii than those in an enzymic hydrolysate. Threonine and serine were most actively degraded, but no resultant increase in growth yield occurred. Branched-chain volatile fatty acid production, which increased as the dilution rate of a glucose-limited chemostat decreased, seemed to be associated ...
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2002
ISSN: 1364-5072,1365-2672
DOI: 10.1046/j.1365-2672.2002.01580.x