Enhancement of Halophilic Glutaminase Producing by Tetragenococcus muriaticus FF5302 in Bioreactor

From Thai fermented fish sauce (Nam-pla), 59 bacterial isolates of halophilic glutaminase-producing bacteria were isolated. The hydrolysis glutamine served as the primary screening procedure. It was discovered that strain FF5302 an influential producer extracellular glutaminase enzyme. moderately bacterium Tetragenoccus muriaticus identified through sequence analysis 16S rRNA gene, phylogenetic tree analysis, and phenotypic identification before it possible to determine optimal nutritional culture conditions for its activity. purpose this research cultural producing activity in a stirred tank bioreactor with volume capacity 3 L. production from investigated by optimizing various physicochemical parameters. Seven potential factors are generally considered production, namely NaCl concentration, initial pH, temperature, incubation time, nitrogen sources, carbon inoculum size. According findings, amount had effect on growth enzyme when present at concentration 5 % (v/v). also found showed highest (87.4 U mL−1) SGC liquid medium containing 20 (w/v), pH 8.0, agitation 200 rpm, aeration rate 0.05 VVM 37 °C 120 h. size influenced both proliferation inoculum. Consequently, T. possessed exceptional synthesize glutaminase. Furthermore, is prospective option usage food industry aroma flavor enhancer. HIGHLIGHTS exceptionally capable In addition, viable candidate study could be helpful valuable improving productivity scale industrial applications. GRAPHICAL ABSTRACT