Enhanced transgene expression using cis-acting elements combined with the EF1 promoter in a mammalian expression system
نویسندگان
چکیده
منابع مشابه
Selectable Marker Gene Removal and Expression of Transgene by Inducible Promoter Containing FFDD Cis-Acting elements in Transgenic plants
Abstract Background: Selectable marker gene (SMG) systems are critical for generation of transgenic crops. Transgenic crop production Background: Selectable marker gene (SMG) systems are critical for generation of transgenic crops. Transgenic crop production without using SMG is not economically feasible. However, SMGs are non-essential once an intact transgenic plant has been established. Eli...
متن کاملselectable marker gene removal and expression of transgene by inducible promoter containing ffdd cis-acting elements in transgenic plants
abstract background: selectable marker gene (smg) systems are critical for generation of transgenic crops. transgenic crop production background: selectable marker gene (smg) systems are critical for generation of transgenic crops. transgenic crop production without using smg is not economically feasible. however, smgs are non-essential once an intact transgenic plant has been established. elim...
متن کاملCis-acting elements in the antigenomic promoter of Nipah virus.
Genome synthesis in paramyxoviruses, including Nipah virus (NiV), is controlled by sequence elements that reside in the non-coding nucleotides at the 5'-trailer (3'-antigenomic) end that make up the antigenomic promoter (AGP). Using a chloramphenicol acetyl transferase-based plasmid-driven minigenome system, the terminal 96 nt of NiV AGP were first mutagenized in blocks of three hexamers to ena...
متن کاملA rice cab gene promoter contains separate cis-acting elements that regulate expression in dicot and monocot plants.
The major light-harvesting chlorophyll a/b binding proteins of the photosynthetic apparatus are encoded by families of nuclear cab genes. The expression of most cab genes is tissue specific and photoregulated in angiosperms. In transgenic tobacco plants, expression of the reporter gene beta-glucuronidase (GUS) is photoregulated and tissue specific from 5' upstream sequences of the rice cab1R ge...
متن کاملEfficient transgene expression system using a cumate-inducible promoter and Cre-loxP recombination in avian cells
OBJECTIVE Transgenic technology is widely used for industrial applications and basic research. Systems that allow for genetic modification play a crucial role in biotechnology for a number of purposes, including the functional analysis of specific genes and the production of exogenous proteins. In this study, we examined and verified the cumate-inducible transgene expression system in chicken D...
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ژورنال
عنوان ژورنال: European Journal of Pharmaceutical Sciences
سال: 2018
ISSN: 0928-0987
DOI: 10.1016/j.ejps.2018.08.016