Enhanced green fluorescent protein as an alternative control reporter to Renilla luciferase
نویسندگان
چکیده
منابع مشابه
Generation of destabilized green fluorescent protein as a transcription reporter.
The green fluorescent protein (GFP) is a widely used reporter in gene expression and protein localization studies. GFP is a stable protein; this property allows its accumulation and easy detection in cells. However, this stability also limits its application in studies that require rapid reporter turnover. We created a destabilized GFP for use in such studies by fusing amino acids 422-461 of th...
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The introduction of exogenous DNA sequences into cultured cells is a cornerstone in the study of mammalian gene expression. For most cell types, the efficiency of stable gene transfer is usually quite low, which mandates the use of selectable markers to isolate a population of transfected cells with the desired phenotype. In simple gene expression experiments, only one type of selectable marker...
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Enzymes are deformable molecules: the molecular structure can exist in essentially a continuum of different conformations. These are accessible by applying mechanical stress to the molecule. Mechanics then provides a general molecular mechanism to modulate enzymatic reactions, by applying mechanical stresses which deform the enzyme. There are approximately 10 known different enzymatic reactions...
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BACKGROUND Reporter genes are often used as a selectable marker for generation of recombinant viruses in order to investigate the mechanism of pathogenesis and to obtain candidate vaccine viruses. Routine selection of the recombinant parapoxvirus is time-consuming and labor intensive. Therefore, developing a novel method for selection is critical. RESULTS In this study, we developed a rapid m...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2005
ISSN: 0003-2697
DOI: 10.1016/j.ab.2005.04.047