Enhanced Anticancer Effect of Temozolomide through Synergistic Combination with Diltiazem in Neuroblastoma (SH-SY5Y) Cell Line
نویسندگان
چکیده
Objective: This study aimed to assess the enhanced anticancer effect of Temozolomide (TMZ) through a synergistic combination with Diltiazem (DTZ) in neuroblastoma cell line (SH-SY5Y).
 Material and Methods: The SH-SY5Y was cultured DMEM medium. TMZ DTZ stock solutions were prepared diluted obtain different concentrations. XTT assay performed evaluate viability. Cells treated alone or for 24 48 hours. Viability measured using XTT-formazan product, statistical analysis performed.
 Results: viability cells TMZ+DTZ assessed. At hours, showed no cytotoxic effect, while low impact. exhibited significant effects. effects concentration-dependent time-dependent. on at specific concentrations.
 Conclusion: demonstrated that combined had cells. may enhance overcome drug resistance treatment. DTZ, P-glycoprotein inhibitor, could prevent efflux from cancer cells, increasing its concentration effectiveness.
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ژورنال
عنوان ژورنال: Cumhuriyet medical journal
سال: 2023
ISSN: ['1300-1957', '1305-0028']
DOI: https://doi.org/10.7197/cmj.1313269