Engineering Microsomal Cytochrome P450 2C5 to Be a Soluble, Monomeric Enzyme
نویسندگان
چکیده
منابع مشابه
Engineering Bacterial Cytochrome P450 Bm3 into a Prototype with Human P450 Enzyme Activity Using Indigo Formation
School of Biological Sciences and Technology, Chonnam National University, Gwangju 500757, Republic of Korea (S.-H.P., Do.-H.K., Da.-H.K., C.-H.Y.); Systems Microbiology Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305806, Republic of Korea (Doo.K., H.-C.J., J.-G.P.); Department of Biochemistry, College of Veterinary Medicine, Chonnam National University, G...
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Protein engineering of cytochrome P450 monooxygenases (P450s) has been very successful in generating valuable non-natural activities and properties, allowing these powerful catalysts to be used for the synthesis of drug metabolites and in biosynthetic pathways for the production of precursors of artemisinin and paclitaxel. Collected experience indicates that the P450s are highly 'evolvable' - t...
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A cytochrome P450 (CYP) enzyme, 3'-daidzein hydroxylase, CYP105D7 (3'-DH), responsible for daidzein hydroxylation at the 3'-position, was recently reported. CYP105D7 (3'-DH) is a class I type of CYP that requires electrons provided through electron transfer proteins such as ferredoxin and ferredoxin reductase. Presently, we constructed an artificial CYP in order to develop a reaction host for t...
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Human cytochrome P450 (P450) enzymes metabolize a variety of endogenous and xenobiotic compounds, including steroids, drugs, and environmental chemicals. In this study, we examine the possibility that bacterial P450 BM3 (CYP102A1) mutants with indole oxidation activity have the catalytic activities of human P450 enzymes. Error-prone polymerase chain reaction was carried out on the heme domain-c...
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Cultured human hepatocytes are a valuable in vitro system for evaluating new molecular entities as inducers of cytochrome P450 (P450) enzymes. The present study summarizes data obtained from 62 preparations of cultured human hepatocytes that were treated with vehicles (saline or dimethylsulfoxide, 0.1%), beta-naphthoflavone (33 microM), phenobarbital (100 or 250 microM), isoniazid (100 microM) ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2000
ISSN: 0021-9258
DOI: 10.1074/jbc.275.4.2545