Engineering Antibody Fitness and Function Using Membrane-Anchored Display of Correctly Folded Proteins

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Targeting membrane proteins for antibody discovery using phage display

A critical factor in the successful isolation of new antibodies by phage display is the presentation of a correctly folded antigen. While this is relatively simple for soluble proteins which can be purified and immobilized onto a plastic surface, membrane proteins offer significant challenges for antibody discovery. Whole cell panning allows presentation of the membrane protein in its native co...

متن کامل

Membrane-anchored chemokine fusion proteins

Adoptive or active cancer immunotherapy can fail owing to the inefficient recruitment of effector leukocytes to malignant lesions. The intratumoral injection of recombinant proteins comprising a chemokine-derived domain linked to the mucin stalk of chemokine (C-X3-C motif) ligand 1 (CX3CL1) and a glycosylphosphatidylinositol anchor can specifically enhance the recruitment of effector cell subse...

متن کامل

Pathway Leading to Correctly Folded β-Tubulin

*Department of Biochemistry noff et al., 1989; Ostermann et al., 1989; Martin et al., New York University Medical Center 1991; Gething and Sambrook, 1992; Gao et al., 1992; New York, NY 10016 Horwich et al., 1993; Lewis et al., 1996). †Flanders Interuniversity Institute for Biotechnology It has not provedpossible to obtain exchange-compeUniversity of Ghent tent tubulin following denaturation of...

متن کامل

Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins.

Glycosylphosphatidylinositol-anchored proteins (GPI-APs) are a functionally and structurally diverse family of post-translationally modified membrane proteins found mostly in the outer leaflet of the plasma membrane in a variety of eukaryotic cells. Although the general role of GPI-APs remains unclear, they have attracted attention because they act as enzymes and receptors in cell adhesion, dif...

متن کامل

Antibody affinity maturation using yeast display with detergent-solubilized membrane proteins as antigen sources.

Antigen preparations in the form of detergent-solubilized cell lysates could, in principle, render membrane proteins (MPs) compatible with in vitro antibody engineering technologies. To this end, detergent-solubilized cell lysates were coupled with the yeast surface display platform to affinity mature an anti-transferrin receptor (TfR) single-chain antibody (scFv). Lysates were generated from T...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Journal of Molecular Biology

سال: 2012

ISSN: 0022-2836

DOI: 10.1016/j.jmb.2011.12.021