Endpoint Colorimetric Method for Assaying Total Cholesterol in Serum with Cholesterol Dehydrogenase

Endpoint colorimetric method for assaying total cholesterol in serum with cholesterol dehydrogenase.

BACKGROUND Various methods are available to measure serum cholesterol concentrations. Of these, the cholesterol ester hydrolase (CEH)-cholesterol oxidase-peroxidase chromogenic method is widely used. However, this method has the disadvantage of interference by reducing substances. We developed and evaluated an endpoint assay for serum cholesterol, based on a CEH-cholesterol dehydrogenase (CDH)-...

A simple method for the determination of serum cholesterol

Reference Values for Serum Total Cholesterol Concentrations Using Percentile Regression Model: A Population Study in Mashhad

Background and Purpose: Serum total cholesterol (TC) concentrations are affected by several factors including ethnicity, diet, geographic, and environmental determinants, and are related to another disease, including hypothyroidism, and renal and liver disease. It is associated with an increased risk of cardiovascular disease, particularly if associated with high levels of serum low-density lip...

The remonal effect of hydrogenated shortening on serum levels of triglycerids,total cholesterol and HDL-cholesterol in normal subjects

In Iran,the hydrogenated vegetable oil have content of frans fatty acids and high per capita consumption.this study was aimed to evaliate the effects of substitution of hydrogenated oil by sunflower liquid oil on plasma levels of LDL,HDL, total cholestero and triglycerids in normal subjects.thirty six healthy inmates participated in this research. they were consuming hydrogenated oil before the...

A rapid and specific ultramicro method for total serum cholesterol.

A modification of the Zak cholesterol reaction is described in which ethanol is substituted for glacial acetic acid. This modification avoids the hazard of purifying glacial acetic acid and also increases the specificity of the reaction by eliminating ir.terference from bromide or iodide salts. The ethanolic reaction has the absorbance spectrum, sensitivity, and stability of the usual acetic ac...