Endothelial cell transfection of ex vivo arteries

Ex vivo adenovirus mediated gene transfection of human conjunctival epithelium.

AIM To investigate the efficacy of "ex vivo" adenoviral vector mediated gene transfection of human conjunctival epithelial cell as a possible route for gene therapy for the distribution of anti-inflammatory agents for the potential treatment of immune mediated ocular inflammatory disorders. METHODS Human conjunctival cells (HCs) were cultured with various concentrations of recombinant adenovi...

Ultrasonically targeted delivery into endothelial and smooth muscle cells in ex vivo arteries.

This study tested the hypothesis that ultrasound can target intracellular uptake of drugs into vascular endothelial cells (ECs) at low to intermediate energy and into smooth muscle cells (SMCs) at high energy. Ultrasound-enhanced delivery has been shown to enhance and target intracellular drug and gene delivery in the vasculature to treat cardiovascular disease, but quantitative studies of the ...

Ex Vivo Behaviour of Human Bone Tumor Endothelial Cells

Cooperation between endothelial cells and bone in bone remodelling is well established. In contrast, bone microvasculature supporting the growth of primary tumors and metastasis is poorly understood. Several antiangiogenic agents have recently been undergoing trials, although an extensive body of clinical data and experimental research have proved that angiogenic pathways differ in each tumor t...

Cell-based methods for ex vivo evaluation of human endothelial biology.

The endothelium plays a central role in cardiovascular regulation. Endothelial cells produce a variety of vasculoregulatory and vasculotropic molecules that act locally or at distant sites. Alteration of the vascular endothelium is a primary event in the pathogenesis of vascular diseases, such as atherosclerosis, as well as systemic and pulmonary hypertension. For these reasons, the study of hu...

Ex Vivo Cytokine and Memory T Cell