Endo-1,4-D-glucanohydrolase assisted extraction of essential oil from the seed kernels of Nutmeg by using a two-step protocol

The cell walls of plants are made up cellulose as the major composite and hydrolysis this polysaccharide has proven to be a step in extraction many biomolecules. This research was on both pure partially purified endo-1,4-D-glucanohydrolase, EC 3.2.1.4 (cellulase) assisted essential oil from seed kernels Nutmeg by using two-step protocol that involved pretreatment sample with cellulase followed distillation Clevenger apparatus. obtained after 8.2 % while 5.8 when treatment prior hydro-distillation done cellulase. An yield 3.8 observed no enzyme carried out. GC-MS analysis showed presence 25 24 components pretreated respectively. There were three groups oils. These monoterpenes sabinene, α-pinene, β-pinene; sesquiterpenes safrole alpha-copaene; phenylpropanoid/aromatic compounds, composed myristicin methyl eugenol. these principal compounds given nutmeg improved value due possibility incorporating them functional ingredients several products possible applications pharmaceutical, agricultural, fragrance, flavour, cosmetic industries