Enantioanalysis of Leucine in Whole Blood Samples Using Enantioselective, Stochastic Sensors

Haemoglobin electrophoresis on cellulose acetate using whole blood samples.

A modification of the method of haemoglobin electrophoresis using cellulose acetate membrane described by Kohn (1969) enables whole blood to be applied direct to the membrane instead of a haemolysate. The method gives results which are indistinguishable from those obtained using a haemolysate. Normal protein stains are not recommended, because of interference from plasma proteins, but the metho...

Homogeneous immunoassay of whole-blood samples.

Proof of principle has been shown for a rapid, quantitative, homogeneous immunoassay capable of analyzing whole-blood samples. The assay was performed with test cards and a small instrument designed for use at the point of care. The immunoassay has an immunological "front end" combined with a coagulation cascade chemistry "back end" and is made possible by combining two patented technologies: (...

Development of the rapid and simplified ELISA (WHOLE BLOOD-ELISA) using samples of Schistosoma japonicum-infected human whole blood.

An ELISA technique was developed using samples of Schistosoma japonicum-infected human whole blood based on the conventional ELISA. In this study, the following were demonstrated. 1) Whole blood samples could be used. 2) The volume of whole blood and conjugate could be reduced to 0.05 ml. 3) The incubation time was shortened to 5 minutes. 4) The optical density could be measured at 10 minutes a...

Detection of Cytomegalovirus DNA in EDTA Whole Blood Samples:

Stabilization of mRNA expression in whole blood samples.

BACKGROUND Accurate quantification of mRNA in whole blood is made difficult by the simultaneous degradation of gene transcripts and unintended gene induction caused by sample handling or uncontrolled activation of coagulation. This study was designed to compare a new blood collection tube (PAXgene Blood RNA System) and a companion sample preparation reagent set with a traditional sample collect...