Embryotoxicity of Yangambin Isolated from Ocotea duckei Vattimo-Gil in Gallus gallus domesticus Embryos

Pharmacognosy Research,2021,13,3,135-139.DOI:10.5530/pres.13.3.5Published:June 2021Type:Original ArticleAuthors:Thiago Felix da Silva, Maria Isabel de Assis Lima Castro, Marta Gerusa Soares Lucena, Camilla Vila Nova Laisla Rangel Peixoto, Eliane Alves Bandeira Carvalho, Silvania Tavares Paz, Beathryz Lethycya Almeida Guimarães, José Barbosa Filho, and Eliete Cavalcanti Silva Author(s) affiliations:Thiago Silva1,*, Castro2, Lucena3, Silva4, Peixoto5, Carvalho6, Paz7, Guimarães3, Filho8, Silva1 1Postgraduate Program in Morphotechnology - Federal University of Pernambuco, Recife, PE, BRAZIL. 2Master Sciences; Postgraduate Biological Sciences 3Department Histology Embryology 4Multiprofessional Residency Health Surveillance Recife Department, 5Master Institute Drugs Medicines Research Paraíba, João Pessoa, PB, 6Master 7Master Department Pathology 8Institute Abstract:Introduction: Yangambin, a lignan predominant the leaves Ocotea duckei Vattimo-Gil, has several biological activities, such as anti-convulsant, analgesic, anti-inflammatory leishmanicidal. Objectives: The aim this study was to evaluate embryotoxicity yangambin on neurodevelopment Gallus gallus domesticus embryos. Materials Methods: 120 fertilized eggs were divided three groups: G1 (PBS with 0.1 % Tween 80), G2 (50 μg/ml yangambin) G3 (65 each egg inoculated 100 μL respective solutions. incubated at temperature 37.5°C, relative humidity 65% 75%, for 48 hr then their embryos histologically processed. Results: In staging, carried out according Hamburger Hamilton (1951), variations stages identified. all groups, morphological analysis revealed closure anterior neuropore absence malformations optic vesicles secondary encephalic vesicles. caudal region, standard development neural tube observed, well-segmented somites regression primitive line. cross sections showed that internal structure somite’s, composed dermatome, myotome sclerotome, preserved. statistical did not show significant differences between groups regarding morphometry cephalic regions tube. Conclusion: Yangambin embryotoxic effects embryos, under tested conditions. Keywords:Chicken embryo, Lauraceae, Lignan, Neurodevelopment, Secondary metabolitesView:PDF (809.58 KB)