Embryonic stem cell virus, a recombinant murine retrovirus with expression in embryonic stem cells.

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Embryonic stem cell virus, a recombinant murine retrovirus with expression in embryonic stem cells.

The expression of Moloney murine leukemia virus and vectors derived from it is restricted in undifferentiated mouse embryonal carcinoma and embryonal stem (ES) cells. We have developed a retroviral vector, the murine embryonic stem cell virus (MESV), that is active in embryonal carcinoma and ES cells. MESV was derived from a retroviral mutant [PCC4-cell-passaged myeloproliferative sarcoma virus...

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Comparing the Expression Levels of Alkaline Phosphatase, Gfra1, Lin28, and Sall4 Genes in Embryonic Stem Cells, Spermatogonial Stem Cells, and Embryonic Stem-Like Cells in Mice

Background and purpose: Spermatogenesis is a well-organized process that is influenced by a variety of factors. Alkaline phosphatase, and Gfra1, Lin28, and Sall4 genes are among the key players in this interconnected process. This study aimed to investigate the expression levels of Gfra1, Lin28, and Sall4 genes in embryonic, spermatogonial, and embryonic stem-like (ES-like) cells in mice. Mate...

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Evaluation and Comparison of the Expression Levels of the ZBTB16 (Plzf) and ZFP Genes and Alkaline Phosphatase in Three Cell Populations: Mouse Spermatogonial Stem Cells, Embryonic Stem-Like Cells (Es-Like), And Embryonic Stem Cells

Introduction: One of the vital enzymes during spermatogenesis, which is one of the pluripotency factors of stem cells and contributes to maintaining their pluripotency is alkaline phosphatase. ZBTB16 and ZFP proteins are critical elements in stem cells which are expressed in pluripotent stem cells and maintain their pluripotency due to their role in messaging pathways. Material & Methods: The ...

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Extract of mouse embryonic stem cells induces the expression of pluripotency genes in human adipose tissue-derived stem cells

Objective(s): In some previous studies, the extract of embryonic carcinoma cells (ECCs) and embryonic stem cells (ESCs) have been used to reprogram somatic cells to more dedifferentiated state. The aim of this study was to investigate the effect of mouse ESCs extract on the expression of some pluripotency markers in human adipose tissue-derived stem cells (ADSCs). Materials and Methods: Human A...

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Embryonic stem cell-derived hematopoietic stem cells.

Despite two decades of studies documenting the in vitro blood-forming potential of murine embryonic stem cells (ESCs), achieving stable long-term blood engraftment of ESC-derived hematopoietic stem cells in irradiated mice has proven difficult. We have exploited the Cdx-Hox pathway, a genetic program important for blood development, to enhance the differentiation of ESCs along the hematopoietic...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1990

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.87.23.9202