Embryo Morphokinetic Activity Evident in Short Videos of In Vitro Bovine Embryos

Embryo transfer (ET) and in vitro fertilization (IVF) are increasing use by dairy producers as a means to breed their animals these assisted reproductive techniques can optimize the genetics of or enable “beef on dairy” programs increase profitability dairy. Due advantages ET IVF, it is anticipated that will continue despite status underwhelmingly low pregnancy outcomes. Pregnancy rates bovine ET/IVF remain below 56%, with many implementing beef reporting be lower than 23%. The inability objectively evaluate embryo health prior into recipient contributing factor this problem 20% transferred embryos inviable at time have little chance establishing pregnancy. objective research was real-time morphokinetic activity based 30 s video recordings day 7.5 morulas correlate developmental Eighty-eight were recorded standard culture conditions an SMZ-1000 Stereo zoom microscope TE-300 Nikon inverted microscope. difference embryo’s measured frame-by-frame correlated hatching It found demonstrated higher outcomes, suggesting measurement noninvasive non-subjective method competency used improve efficiency IVF/ET cattle.