Embryo-endometrium crosstalk: a new understanding from in vitro model

Embryo-endometrium crosstalk has a pivotal role during implantation. However, it is difficult to reveal the actual events and mechanisms involved in because of ethical technical limitations. On one hand, almost impossible conduct such experiments on human beings. other challenging establish an embryo-endometrium model mimic vivo process vitro. Endometrial glandular organoid culture been developed recently (1Turco M.Y. Gardner L. Hughes J. Cindrova-Davies T. Gomez M.J. Farrell et al.Long-term, hormone-responsive cultures endometrium chemically defined medium.Nat Cell Biol. 2017; 19: 568-577Crossref PubMed Scopus (200) Google Scholar). The organoids are 3D spherical structures formed from self-organizing endometrial epithelial cells can be expanded for long time. current recapitulate most molecular functional characteristics epithelium. With modification original protocol, Dr. Piomboni coworkers (2Luddi A. Pavone V. Semplici B. Goverini Criscuoli M. Paccagnini E. al.Organoids endometrium: powerful vitro endometrium-embryo cross-talk at implantation site.Cells. 2020; 9: 1121Crossref (11) Scholar) previously established eutopic endometrium. In their study, they showed that exhibited features mimicking cells/tissue origin. First, displayed organization cellular ultrastructural as do vivo. Second, responded treatment with steroids which simulate hormonal milieu menstrual cycle proliferative phase-induced secretory morphology gene expressions similar those found primary tissue. Third, markers receptivity, including development pinopodes expression glycodelin-A, environment simulating mid-secretory phase. this issue Fertility Sterility, same group took advantage continued work investigating interactions (3Luddi Governini Capaldo Landi C. Letta F. al.Emerging embryo secretome paracrine communication site: proof concept.Fertil Steril. 2021; 115: 1054-1062Abstract Full Text PDF (2) cultured spent medium embryos up Day 3. conditioned was expected contain products embryonic results induced change glycodelin-A terms increase abundance well glycoform pattern. also compared fertilization (IVF) outcome patients received 3 transfer presence or fresh medium. They significant biochemical clinical pregnancy rate when loaded (17.5% vs. 36.6% 16.5% 35.1%, respectively; P<.05). We thank authors contribution literature. study provides evidence molecule(s) could facilitate via modulating receptivity. echo previous showing association certain cell-free microRNAs implanted euploid blastocysts (4Capalbo Ubaldi F.M. Cimadomo D. Noli Khalaf Y. Farcomeni al.MicroRNAs blastocyst derived trophectoderm explored reproductive competence assessment.Fertil 2016; 105: 225-235Abstract (83) embryo-derived potential use biomarker hot research topics. An exciting novel observation improved IVF inclusion transfer. It represents simple practical method enhancing performance. Although great interest, several questions remain unanswered. unclear active components affect Nowadays, these identified using omics technologies. identification not only academic interest but potentially used infertility due factors. factors affecting known. will interesting know if production associated (cleavage-stage blastocyst), developmental competence, karyotype. More importantly, non-invasive should investigated. concept increases meaningful. But sample size insufficient. needs substantiated properly powered designed randomized controlled trials. From physiological perspective, instead cleavage-stage considered. Fourth, regulation formation system glands. pinopodes, luminal feature, may enable us differentiation epithelium This opens door investigate aspects embryo-endometrial At time, calls more done. Nevertheless, our field right track building knowledge toward optimizing treatment. Emerging conceptFertility SterilityVol. 115Issue 4PreviewTo assess modifying profile glycodelin A (GdA) (ORG) window. To verify whether embryo-conditioned time outcome. Full-Text