منابع مشابه
Adipose Tissue LPL Activity and Fat Cell Size Activity per weight Activity per cell Elution Elution Detergent Elution Elution Detergent
In obese women (a = 16) at their usual weight, fasting adipose tissue lipoprotein lipase (LPL) activity, obtained by elution with serum and heparin at 40 and 370C, was inversely correlated to plasma estradiol levels (r = -0.724; P = 0.002) and (r = -0.641; P = 0.010), respectively. Furthermore, fasting postheparin plasma LPL activity during a heparin infusion, showed an even stronger inverse co...
متن کاملElution patterns of rubella IgM, IgA, and IgG antibodies from a dextran and an agarose gel.
The elution pattern of serum proteins and the distribution of rubella HAI activity in 95 sera from 65 cases were determined after gel filtration with (a) Sephadex G-200 and (b) Bio-Gel A-5M. Rubella HAI antibody in peak 1 after Sephadex G-200 fractionation of early convalescent sera consists of both IgM and high molecular weight IgA. However, these two classes of antibody can be distinguished b...
متن کاملBiospecific Elution from Non-Specific Adsorbents (‘Affinity Elution’)
Enzymes adsorbed to ion exchangers are in many cases specifically desorbed by interaction with their ligands. This phenomenon has been variously called ‘specific elution with substrate’ or ‘affinity elution’ in the literature (von der Haar, 1974). Because the most effective ligands for elution are those containing charged groups identical with those on the ion exchanger (positive groups in the ...
متن کاملElution of antibodies from a Protein-A column by aqueous arginine solutions.
Acidic pH is commonly used to elute antibodies from Protein-A affinity column, although low pH may result in aggregation of the proteins. As an alternative, here arginine was tested as an eluent and compared with a more conventional eluent of citrate. Using purified monoclonal antibodies, recovery of antibodies with 0.1M citrate, pH 3.8, was less than 50% and decreased further as the pH was inc...
متن کاملSoft-tissue Biopsy Technique
The instrument described here consists of a special syringe on which sharp cutting needles of various sizes are mounted. These produce undistorted biopsy specimens. Suction is used only to retain the specimen within the needle, and the cleancut cylinder of tissue is quite unlike that obtained by aspiration. Gillman and Gillman&2 used this principle for their liver biopsy instrument. We have ret...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1970
ISSN: 0021-9746
DOI: 10.1136/jcp.23.7.629