Elevated Glutathione Biosynthetic Capacity in the Chloroplasts of Transgenic Tobacco Plants Paradoxically Causes Increased Oxidative Stress
نویسندگان
چکیده
منابع مشابه
Elevated glutathione biosynthetic capacity in the chloroplasts of transgenic tobacco plants paradoxically causes increased oxidative stress
Glutathione (GSH), a major antioxidant in most aerobic organisms, is perceived to be particularly important in plant chloroplasts because it helps to protect the photosynthetic apparatus from oxidative damage. In transgenic tobacco plants overexpressing a chloroplast-targeted gamma-glutamylcysteine synthetase (gamma-ECS), foliar levels of GSH were raised threefold. Paradoxically, increased GSH ...
متن کاملEnhancement of oxidative stress tolerance in transgenic tobacco plants overproducing Fe-superoxide dismutase in chloroplasts.
A chimeric gene consisting of the coding sequence for chloroplastic Fe superoxide dismutase (FeSOD) from Arabidopsis thaliana, coupled to the chloroplast targeting sequence from the pea ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit, was expressed in Nicotiana tabacum cv Petit Havana SR1. Expression of the transgenic FeSOD protected both the plasmalemma and photosystem II against...
متن کاملIncreased resistance to oxidative stress in transgenic plants by targeting mannitol biosynthesis to chloroplasts.
To investigate the potential role of a polyol, mannitol, in oxidative stress protection, a bacterial mannitol-1-phosphate dehydrogenase gene was targeted to chloroplasts by the addition of an amino-terminal transit peptide. Transgenic tobacco (Nicotiana tabacum) lines accumulate mannitol at concentrations ranging from 2.5 to 7 mumol/g fresh weight. Line BS1-31 accumulated approximately 100 mM m...
متن کاملDehydrins Impart Protection against Oxidative Stress in Transgenic Tobacco Plants
Environmental stresses generate reactive oxygen species (ROS) which might be detrimental to the plants when produced in an uncontrolled way. However, the plants ameliorate such stresses by synthesizing antioxidants and enzymes responsible for the dismutation of ROS. Additionally, the dehydrins were also able to protect the inactivation of the enzyme lactate dehydrogenase against hydroxyl radica...
متن کاملProtection from oxidative stress in transgenic plants.
37 Karpinksi. S. (1994) I’h.1). Thesis. Swedish University of Agricultural Sciences. limea Greer. S. and I’erham, K. N. (1986) Biochemistry 25. 2736-2742 Kunert, K. J., Cresswell. C. F., Schmidt. A.. Mullineaux, 1’. M. and Foyer, C. H. (1990) Arch. Hiochem. Hiophys. 282.233-238 Foyer. C. H.. Lelandais. M., Galap, C. and Kunert, K. J. (1991) I’lant I’hysiol. 97.803-872 Aono. M., Kubo. A., Saji9 ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Plant Cell
سال: 1999
ISSN: 1040-4651,1532-298X
DOI: 10.1105/tpc.11.7.1277