Electrospun fibrous sponge via short fiber for mimicking 3D ECM

Abstract Background Most of the natural extracellular matrix (ECM) is a three-dimensional (3D) network structure micro/nanofibers for cell adhesion and growth 3D. Electrospun fibers distinctive mimicked 2D ECM, however, it impossible to simulate 3D ECM because longitudinal collapse continuous micro/nanofibers. Herein, electrospun micro/nano-fibrous sponge was fabricated via electrospinning, homogenization, shaping thermal crosslinking tissue regeneration cells vascular. Results Fibrous exhibited high porosity, water absorption compression resilience no chemical crosslinked agent used in preparation process. In vitro studies showed that short fiber provided an oxygen-rich environment growth, which conducive proliferation HUVECs, stimulated expression VEGF, well promoted vascularization HUVECs. vivo sponges had good chronic wound diabetes rats. Furthermore, fibrous were better than micro/nanofiber membranes promoting repair diabetic full-thickness skin defects including healing, hair follicle regeneration, angiogenesis, collagen secretion. Conclusion Therefore, are special candidates mimicking tissue. Graphic