Electroporation, an alternative to biolistics for transfection of Bombyx mori embryos and larval tissues
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چکیده
منابع مشابه
Electroporation, an alternative to biolistics for transfection of Bombyx mori embryos and larval tissues
There are few powerful techniques available to transfect insect tissues. We previously used biolistics to transfect Bombyx mori embryos, and larval and pupal tissues (Thomas J-L et al. 2001. Journal of Insect Science 1/9, Kravariti L et al. 2001. Insect Biochemistry and Molecular Biology 31: 473-479). As the main limitation was the irregularity in results we explored electroporation as an alter...
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15 صفحه اولEvidence for a low-affinity, high-capacity uniport for amino acids in Bombyx mori larval midgut.
We investigated the kinetics of leucine influx as a funtion of external substrate concentration between 0.03 and 16 mM in brush-border membrane vesicles (BBMV) prepared from the middle region of Bombyx mori larval midgut. A detailed kinetic analysis of leucine uptake led to the identification, in parallel with the K+-dependent symporter for neutral amino acids, of a K+-independent, low-affinity...
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ژورنال
عنوان ژورنال: Journal of Insect Science
سال: 2003
ISSN: 1536-2442
DOI: 10.1093/jis/3.1.17