Electrophoretic separation of creative kinase isozyme
نویسندگان
چکیده
منابع مشابه
Improved electrophoretic separation of creatine kinase isoenzymes.
Creatine kinase isoenzymes are separated electrophoretically on cellulose acetate, with use of an improved procedure for reactivation and visualization of enzymic activity. N-Acetyl cysteine is used as the reactivator and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide for visualization in a tetrazolium-coupled system. This test, both practical and reliable, is a suitable alter...
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We compared two techniques for separating and evaluating serum creatine kinase isoenzymes--fluorometric agarose electrophoresis and Sephadex chromatography--in 50 patients, 25 of whom had confirmed acute myocardial infarction. In every case isoenzyme MB (heart isoenzyme) was detected with equal sensitivity by either procedure. Evidently, only the presence or absence of MB is clinically signific...
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Since 1991, the Ontario Laboratory Proficiency Testing Program has assessed the analytical performance of creatine kinase (CK; EC 2.7.3.2) isoenzyme-2, using fresh human serum supplemented with purified human CK isoenzymes. In Ontario, the 142 laboratories licensed to analyze CK-2 use a variety of methods: electrophoresis-based, immunoinhibition, and mass assays. During a 1992 survey, duplicate...
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In the present work, CdSe nanocrystals (NCs) synthesized with a trioctylphosphine surface passivation layer were modified using amphiphilic molecules to form a surface bilayer capable of providing stable NCs aqueous solutions. Such modified nanocrystals were used as a test solute in order to analyze new electrophoretic phenomena, by applying a micellar plug as a separation tool for discriminati...
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A number of chromatographic methods have been previously reported for the separation of phosphatides. Column chromatography has been used successfully by Thannhauser and Setz (l), Klenk (a), and Hanahan et al. (3). Paper chromatographic techniques for phosphatide analysis have been reported by Bevan et al. (4), Hecht and Mink (5), Hack (6)) and Lea et al. (7). Garvin (8) has reported a separati...
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ژورنال
عنوان ژورنال: SEIBUTSU BUTSURI KAGAKU
سال: 1965
ISSN: 0031-9082,1349-9785
DOI: 10.2198/sbk.11.163