Electrophoretic karyotypes of Phaffia rhodozyma strains
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چکیده
منابع مشابه
Astaxanthin production by Phaffia rhodozyma using a pH-stat control
Fed-batch essays using yeast Phaffia rhodozyma were performed to obtain high astaxanthin concentrations. The yeast was grown over an inexpensive media obtained from the Yucca fillifera date. Feeding was carried out through implantation of a pH-stat control system. Biomass (28.7 mg/l) and pigment (18 mg/l) concentration three folded those that were obtained by previous batch cultures.
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Chromosome-sized DNA molecules of clinical isolates of Torulopsis glabrata were resolved by a pulsed-field electrophoretic method, contour-clamped homogeneous electric fields. With the conditions established in this study, 8 to 12 bands (ranging from 445 to 3,000 kilobases) were observed. There were differences in the intensities and migrations of bands, consistent with T. glabrata being either...
متن کاملStudy of the expression of carotenoid biosynthesis genes in wild-type and deregulated strains of Xanthophyllomyces dendrorhous (Ex.: Phaffia rhodozyma).
The expression, at the mRNA level, of carotenoid biosynthetic genes from Xanthophyllomyces dendrorhous was studied by RT-PCR. The experimental conditions for the RT-PCR assay were standardized to quantify the relative transcript levels of idi, crtE, crtYB and crtI genes. This work attempted to correlate astaxanthin production with the transcript levels of carotenogenic genes in a wild-type stra...
متن کاملThe effect of gamma irradiation on astaxanthin synthetase encoding gene in two mutant strains of Phaffia rhodozyma
BACKGROUND AND OBJECTIVES Astaxanthin, an orange-red carotenoid pigment, acts as a protective agent against oxidative damage to cells in vivo. The astaxanthin synthetase gene (crtS) size consists of 3995 bp. This gene has been suggested to catalyse β-carotene to astaxanthin in Phaffia rhodozyma. The aim of this research was to find any possible changes in this gene in two mutant strains, Gam1 a...
متن کاملSelection of Astaxanthin-Overproducing Mutants of Phaffia rhodozyma with beta-Ionone.
beta-Ionone, an end ring analog of beta-carotene, inhibits astaxanthin production in the red yeast Phaffia rhodozyma. Astaxanthin-overproducing mutants of this yeast are easily spotted on beta-ionone-containing yeast malt agar plates. beta-Ionone appears to block astaxanthin synthesis at the beta-carotene level.
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1994
ISSN: 0378-1097
DOI: 10.1016/0378-1097(94)90211-9