Electrophoretic determination of leptospiral enzymes
نویسندگان
چکیده
منابع مشابه
Pulsed-field gel electrophoretic analysis of leptospiral DNA.
The genomic structures of spirochete species are not well characterized, and genetic studies on these organisms have been hampered by lack of a genetic exchange mechanism in these bacteria. In view of these observations, pulsed-field gel electrophoresis was used to examine the genomes of Leptospira species. Live cells, prepared in agarose plugs, were lysed in situ, and the DNA was analyzed unde...
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This assay procedure for each of the two neuron-specific enolases (alpha gamma and gamma gamma) and the non-neuronal enolase (alpha alpha) in serum involves two steps: electrophoretic separation of the three isoenzymes--alpha alpha, alpha gamma, and gamma gamma--on cellulose acetate, and bioluminescence measurement of total enolase activity. From these data, the activity concentrations (U/L) of...
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Potatoes, members of the Solanaceae plant family, contain calystegines, water-soluble nortropane alkaloids, which are biologically active as glycosidase inhibitors. The content of calystegines A(3) and B(2) in different varieties of potato and in various parts of the tubers (whole potato, peel, flesh, and sprouts) were analysed by new capillary zone electrophoresis and capillary isotachophoresi...
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We describe and evaluate a method for determining isoamylases in serum. With this method, which involves conventional electrophoretic apparatus and commercially available reagents, seven isoenzymes can be resolved. The electrophoresis is performed in agarose gel, and isoenzymes are detected with a water-insoluble cross-linked starch polymer linked to a blue dye. The isoenzymes are quantified by...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1967
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.93.5.1739-1740.1967