منابع مشابه
Electrophoretic behavior of serum amylase.
There have been several indications (1, 2) that the amylase found in serum is derived from sources other than the pancreas or salivary glands. For this reason we wished to determine whether any indication of fractionation of serum amylase could be obtained by paper electrophoresis. Although several accounts of the fractionation of amylase in serum are found in the literature, some involving pre...
متن کاملElectrophoretic behaviour of human urinary amylase.
A saccharogenic method is described for estimating amylase activity in human urine. Results accord with those reported elsewhere except that in this study the peak in the beta zone is a new finding. Comparison between normal and pathological urines suggests that the amylase activity of the beta peak is not of pancreatic origin.
متن کاملElectrophoretic Behavior of Normal Human Serum Albumin at pH 4.0
In the preceding paper on the behavior of pooled normal human serum albumin in various buffers at pH 4.0 (1)) it was demonstrated that the electrophoretic pattern of albumin shows a single moving boundary in the presence of the lower fatty acids, excepting formic and acetic acid, and of certain diand tribasic organic acids. This phenomenon is probably a consequence of the interaction between th...
متن کاملElectrophoretic pattern of amylase isoenzymes in serum and urine of normal persons.
We separated and measured amylase isoenzymes in the serum and urine of 3036 normal persons by electrophoresis on a thin layer of polyacrylamide gel. We wished to establish the normal pattern of these isoenzymes and to evaluate the usefulness of this method of electrophoresis in clinical diagnosis. Results for patients with hyper- or hypofunctioning pancreas and salivary glands suggested that es...
متن کاملSensitive, simple staining method for use in electrophoretic determination of amylase isoenzymes in serum.
We have developed a sensitive and simple staining method for use in electrophoretic analysis of serum alpha-amylase (EC 3.2.1.1) isoenzymes. The principle of this method is as follows. alpha-Amylase hydrolyzes 4-nitrophenylmaltoheptaoside to generate oligosaccharide, which is then converted to gluconolactone in the presence of oligosaccharide dehydrogenase (no EC no. assigned), with concomitant...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1959
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)70177-4