ELECTROPHORETIC ANALYSIS OF PLASMA AND URINARY PROTEINS

Electrophoretic Analysis of Plasma and Urinary Proteins.

Accurate analysis of the protein fractions of human plasma has in the past been very difficult and time-consuming. Fractional precipitation by neutral salt, as in Howe's method (1), has proved useful because of its simplicity and because the most important pathologic variations in plasma proteins involve a gross diminution in the albumin fraction. Such methods have definite limitations: Butler'...

Preliminary treatment of urinary proteins improves electrophoretic analysis and immunodetection.

Analysis of urinary protein composition is an important tool in studies on renal physiology and physiopathology. Urine is, however, a complex mixture containing, besides protein, a variety of compounds such as salts, peptides, oligosaccharides, and glycosaminoglycans. Some of these compounds interfere with the electrophoretic migration of protein in sodium dodecyl sulfate-polyacrylamide gels an...

Electrophoretic Analysis of the Proteins of Plasma and Serous Effusions.

Electrophoretic analysis of serum and urinary proteins in the diagnosis of myelomatosis.

Electrophoresis, using either the classical method of Tiselius or zone electrophoresis on paper, has been widely employed in the study of serum and urinary proteins in myelomatosis, but so far few investigators have examined the diagnostic value of this procedure. In an attempt to make such an assessment, we have compared critically the results of electrophoretic analyses of serum and urinary p...

A study of the Electrophoretic Pattern of the serum Proteins