Electron microscopy of simian virus 40 DNA configuration under denaturation conditions

Fate and expression of simian virus 40 DNA after introduction into murine cells under nonselective conditions.

When SV40 infects mouse cells, it does not replicate but instead causes neoplastic transformation of a small percentage of the cells. It is unknown, however, what happens to the virus in those cells that do not become transformed. We introduced SV40 into mouse cells by nonselective means, either by cotransfection of SV40 DNA with a selectable marker or by random cloning of SV40-infected cells. ...

Electron Microscopy of Fowl Pox Virus

Simian virus 40 integration site in an adenovirus 7-simian virus 40 hybrid DNA molecule.

The E46(+) strain of Adenovirus 7 is a mixed-virus population containing defective Adenoviurs 7-SV40 hybrid particles and helper, nonhybrid Adenovirus 7 particles. We have applied electron microscopic mapping techniques to obtain a physical map of the genome of the hybrid particles present in E46(+)PL1, a substrain of E46(+) derived from a single two-hit plaque. DNA molecules extracted from pur...

Specific Cleavage of Simian Virus 40 DNA by Restriction Endonuclease of Hemophilus Influenzae * ( gel electrophoresis / electron microscopy / DNA mapping / DNA fragments / tumor virus )

A bacterial restriction endonuclease has been used to produce specific fragments of SV40 DNA. Digestion of DNA from plaque-purified stoclrs of SV40 with the restriction endonuclease from Hemophilus influenzae gave 11 fragments resolvable by polyacrylamide gel electrophoresis, eight of which were equimolar with the original DNA. The fragments ranged from about 6.5 X lo5 to 7.4 X lo4 daltons, as ...

Relationship of replication and transcription of Simian Virus 40 DNA.

RNA produced by the Simian Virus 40 (SV40) mutant tsA30 during lytic infection of kidney cells of African green monkeys was examined by RNA-DNA competition-hybridization. This mutant is temperature-sensitive in a function (gene A) that regulates synthesis of viral DNA. No detectable difference between mutant RNA synthesized at the permissive temperature (33 degrees ) and wild-type viral RNA was...