Electrodeposited Gold Nanoparticle (AuNP)-Film as a Nanoplatform for a Label-Free Electrochemical Strongyloidiasis Immunosensor

Strongyloidiasis is an intestinal helminth infection caused by Strongyloides stercoralis . Early detection of this in immunocompromised patients crucial to avoid severe complications and fatality. Herein, we present the potential application electrodeposited AuNP-film developing a label-free electrochemical immunosensor for strongyloidiasis using our synthesized monoclonal antibody. Layer-upon-layer attachment recombinant antibody protein (rMAb23) onto was constructed, utilizing thiol linker via self-assembly monolayer (SAM) technique. The modified electrode utilized detect S. NIE (rNIE) protein. Each successful modification step tested 10 mM [Fe(CN) 6 ] 3−/4− redox couple solution cyclic voltammetric (CV) impedance spectroscopic (EIS) techniques. developed required 20 min incubation with rNIE solution. Specificity study showed no cross-reaction three other proteins. Utilizing EIS measurements on concentration series phosphate-buffered saline (PBS), ranging from 1 μ g mL −1 , obtained limit (LOD) 0.182 also successfully used analyze serum samples individuals healthy people. results indicated that might offer excellent diagnostic capability rapid sensitive antigen strongyloidiasis.