Electrochemical sensing of SARS-CoV-2 amplicons with PCB electrodes

We present a low-cost electrochemical DNA biosensor based on printed circuit board (PCB) electrodes for wastewater monitoring using portable PCR instruments, such as miniPCR®, without the requirement qPCR reagents. PCB are attractive candidates and sensitive biosensors of relevance in pandemic COVID-19, facilitate opportunity to map disease spread Low-Middle Income Countries (LMICs) through environmental samples wastewater. The reported this work is capable detecting amplicons intercalation methylene blue (MB) with DNA, which increases voltammogram peak current at redox potential MB. describe how these changes likely result from adsorption MB-DNA complex electrode surface. reusable, easy clean, do not undergo any surface modification represent cost-effective solution long shelf-life. also explore impact that MB concentration length have upon our performance provide insights useful other investigators field. sensor here SARS-CoV-2 nucleocapsid gene concentrations low 10 pg/?l (approximately 1.7 fM) can detect nucleotides amplified after cycles. Furthermore, approaches described here, were detected simulated sample, by spiking collected sewage treatment plant Mumbai, India RNA.