Electroanalysis of Benzalkonium Chloride in Ophthalmic Formulation by Boron‐doped Diamond Electrode

Benzalkonium chloride (BAK) is a mixture of alkyl benzyl dimethyl ammonium chlorides, which used primarily as biocide, surfactant, preservative, and antimicrobial agent in the pharmaceutical industry, particular ophthalmologic nasal solutions. However, BAK may cause harmful consequences on eye structures anterior segment. Control identity content necessary by applying sensitive detection method. This study unravels use glassy carbon (GC) electrode pristine boron-doped diamond (BDD) for four homologs non-aqueous medium using square wave voltammetry (SWV). The BDD provided more reproducibility oxidation potential than GC with correlation coefficient 0.999. irreversible peak was very broad deconvoluted into 3 peaks corresponding to C12, C14, combined C16–C18 reflect their concentration ratio mixture. method then extended C12 homolog ophthalmic formulations limit (LOD) 0.4 μg/mL. estimated levels three were agreement specified values manufacturers. results validated high-performance liquid chromatography (HPLC) ultraviolet (UV) detection, confirming presence single (C12) drops.