Ehrlichia ruminantium in Amblyomma variegatum and Domestic Ruminants in the Caribbean
نویسندگان
چکیده
منابع مشابه
Rickettsia africae in Amblyomma variegatum and domestic ruminants on eight Caribbean islands.
We used PCRs with omp A primers to determine if spotted fever group rickettsiae occurred in Amblyomma variegatum from 6 Caribbean islands. Positive amplicons were obtained from ticks from the U.S. Virgin Islands (9/18; 50%), Dominica (39/171; 30%), Montserrat (2/5; 40%), Nevis (17/34; 50%), St. Kitts (46/227; 20%), and St. Lucia (1/14; 7%). Sequences for a convenience sample of reaction product...
متن کاملSimultaneous detection of Anaplasma and Ehrlichia species in ruminants and detection of Ehrlichia ruminantium in Amblyomma variegatum ticks by reverse line blot hybridization.
The detection of Anaplasma and Ehrlichia species is usually based on species-specific PCR assays, since no assay is yet available which can detect and identify these species simultaneously. To this end, we developed a reverse line blot (RLB) assay for simultaneous detection and identification of Anaplasma and Ehrlichia species in domestic ruminants and ticks. In a PCR the hypervariable V1 regio...
متن کاملPropagation of the tick Amblyomma variegatum in the Caribbean.
The tropical bont tick, Amblyomma variegatum, is an African tick species which infests livestock and wildlife. It was probably introduced in the central eastern islands of the Caribbean during the 18th or 19th century, with cattle shipped from Senegal. In Africa and the Caribbean, this tick is a vector of heartwater (a rickettsial disease of ruminants) and is associated with acute dermatophilos...
متن کاملVECTOR-BORNE DISEASES, SURVEILLANCE, PREVENTION New Approaches to Detection and Identification of Rickettsia africae and Ehrlichia ruminantium in Amblyomma variegatum (Acari: Ixodidae) Ticks From the Caribbean
Imported from Africa in the 1700s and despite frequent modern eradication efforts, Amblyomma variegatum (F.) spread through the Caribbean by cattle transport, small ruminants, and migrating birds. A. variegatum is a vector for Rickettsia africae, the causative agent of African tick bite fever, and Ehrlichia ruminantium, the causative agent of heartwater. We examined 95 A. variegatum and six Rhi...
متن کاملDifferential transcription of the major antigenic protein 1 multigene family of Ehrlichia ruminantium in Amblyomma variegatum ticks.
The rickettsial pathogen Ehrlichia ruminantium causes heartwater in ruminants and is transmitted by ticks of the genus Amblyomma. The map1 gene, encoding the major surface protein MAP1, is a member of a multigene family containing 16 paralogs. In order to investigate differential transcription of genes of the map1 multigene family in vivo in unfed and feeding ticks, RNA was extracted from midgu...
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ژورنال
عنوان ژورنال: Journal of Medical Entomology
سال: 2011
ISSN: 0022-2585,0022-2585
DOI: 10.1603/me10172