Egg White Avidin
نویسندگان
چکیده
منابع مشابه
Biotin-binding protein from egg yolk. A protein distinct from egg white avidin.
Biotin-binding protein has been purified 2000-fold from chicken egg yolk with a 1 to 2% yield. The puriflcation includes l-butanol extraction of yolk lipids, phosphocellulose chromatography of water-soluble proteins, affinity binding and elution from biotinylSepharose, and gel filtration chromatography. Since the protein biotin-binding sites are saturated with very tightly bound biotin, a novel...
متن کاملThe Concentration and Assay of Avidin, the Injury-producing Protein in Raw Egg White
When the tissues of chicks maintained on a diet containing unheated egg white were found to be deficient in biotin in spite of the abundance of this vitamin in the diet (l), it was concluded that the injury caused by the egg white is not due to any direct toxin but rather is produced indirectly by the action of the egg white in making the biotin of the diet unavailable to the animal. To support...
متن کاملEgg White Proteins
Egg white as a protein system possesses much interest, both biochemical and technological. Study of the egg white proteins has been handicapped by the difficulty of preparation. The five variant system developed by Cohn and coworkers for the fractionation of plasma offers many advantages over the salting-out techniques (1). Low temperatures are employed to improve protein stability. Since low s...
متن کاملEgg white injury.
A 66-year-old man was admitted to our Department for a sudden loss of consciousness, tonic–clonic movement and psychomotor agitation. During the previous 4 months the patient had complained of drowsiness, fatigue, lack of appetite, and nausea. During the prior 2 months he had experienced hair loss, coating of the tongue, and skin flaking with pruritus. Two hours prior to admission, the patient ...
متن کاملHen Egg - white Lysozyme Crystals
Proton tautomerism is a general phenomenon in organic molecules and plays a vital role in many fields of chemistry and biochemistry. The tautomerism of salicylideneanilines [eq(1)] has attracted a considerable attention because it is closely related to thermoand photochromism. Salicylideneanilines greatly favor the enol form over the cis-keto form in the gas phase. We demonstrate here that the ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1971
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)62469-x