Efficient vitrification of mouse embryos using the Kitasato Vitrification System as a novel vitrification device
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چکیده
منابع مشابه
Efficient vitrification of mouse embryos using the Kitasato Vitrification System as a novel vitrification device
BACKGROUND Currently, the cryopreservation of embryos and oocytes is essential for assisted reproductive technology (ART) laboratories worldwide. This study aimed to evaluate the efficacy of the Kitasato Vitrification System (KVS) as a vitrification device for the cryopreservation of mouse embryos to determine whether this novel device can be adapted to the field of ART. METHODS In Experiment...
متن کاملVitrification of Mouse and Sheep Embryos
Vitrification is a method of storing cells in a supercooled state, and is particularly useful for the cryostorage of embryos. With a supply of liquid nitrogen and simple apparatus, vitrification in the field situation is feasible and is potentially simpler and cheaper than the standard freezing processes. However, survival of embryos after vitrification has not yet proved better than after free...
متن کاملWhich stage of mouse embryos is more appropriate for vitrification?
Objective Vitrification has been shown as one of the most effective methods of cryopreservation for mammalian embryos. However, there is no consensus which stage of embryonic development is the most appropriate for vitrification with subsequent maximal development after thawing. This study was carried out to explore and compare the effect(s) of vitrification on mouse 2-cell, 4-cell, 8-cell, mor...
متن کاملVitrification of mouse embryos using the thin plastic strip method
OBJECTIVE The aim of this study was to compare vitrification optimization of mouse embryos using electron microscopy (EM) grid, cryotop, and thin plastic strip (TPS) containers by evaluating developmental competence and apoptosis rates. METHODS Mouse embryos were obtained from superovulated mice. Mouse cleavage-stage, expanded, hatching-stage, and hatched-stage embryos were cryopreserved in E...
متن کاملVitrification of Oocytes and Embryos
Currently, controlled ovarian hyperstimulation protocols commonly provide embryos in excess of those needed for fresh transfer. Therefore, techniques have been developed to store these surplus embryos in liquid nitrogen (referred to as cryopreservation) for an indefinite period of time without significant compromise of their quality. Based on data from the Centers for Disease Control and Preven...
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ژورنال
عنوان ژورنال: Reproductive Biology and Endocrinology
سال: 2017
ISSN: 1477-7827
DOI: 10.1186/s12958-017-0249-2