Efficient production of antibody Fab fragment by transient gene expression in insect cells
نویسندگان
چکیده
منابع مشابه
Cloning and Expression of Simian Rotavirus Spike Protein (VP4) in Insect Cells by Baculovirus Expression System
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Infection of insect cells with baculovirus expression constructs is commonly used to produce recombinant proteins that require post-translational modifications for their activity, such as mammalian proteins. However, technical restraints limit the capacity of insect cell-based culture systems to be scaled up to produce the large amounts of recombinant protein required for human pharmaceuticals....
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Mammalian cell expression systems are currently essential for production of glycosylated biopharmaceuticals such as monoclonal antibodies or molecules requiring even more complex glycan structures. Various host cell and vector systems aimed at improving expression levels and quality have been established (1, 2). Development of biopharmaceutical product candidates from genes to clinical trials s...
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ژورنال
عنوان ژورنال: Journal of Bioscience and Bioengineering
سال: 2017
ISSN: 1389-1723
DOI: 10.1016/j.jbiosc.2017.03.007