Efficient Multifocal Structured Illumination Microscopy Utilizing a Spatial Light Modulator
نویسندگان
چکیده
منابع مشابه
Multifocal multiphoton microscopy based on a spatial light modulator.
We present a new multifocal multiphoton microscope that employs a programmable spatial light modulator to generate dynamic multifocus arrays which can be rapidly scanned by changing the incident angle of the laser beam using a pair of galvo scanners. Using this microscope, we can rapidly select the number and the spatial density of focal points in a multifocus array, as well as the locations an...
متن کاملLive Cell Imaging With Spatial Light Modulator-based Optical Sectioning Structured Illumination Microscopy
Structured illumination microscopy (SIM) is a method in fluorescence microscopy which works by acquiring a set of images using widefield detection. Each image in the set is made with a different position of an illumination mask, but with no mask in the detection path [1]. Subsequent image processing is used to produce an optically sectioned image (OS-SIM) [2 4], or an image with resolution beyo...
متن کاملPlasmonic structured illumination microscopy.
We propose a super resolution imaging technique called plasmonic structured illumination microscopy (PSIM), which combines the structured illumination microscopy technique with the tunable surface plasmon interference. Because of the high-resolution enabled by using surface plasmon interference as an illumination source, PSIM possesses higher image resolving power compared with conventional str...
متن کاملFiber-optic confocal microscopy using a spatial light modulator.
We present a novel fiber-optic confocal microscope in which the scanning operation is achieved by use of a spatial light modulator (SLM) to sequentially illuminate individual fibers or patterns of multiple fibers. Experimental images are presented, and the optical-sectioning capability of the device is demonstrated. The novel SLM-based system is more optically efficient, achieves higher contras...
متن کاملStructured line illumination Raman microscopy
In the last couple of decades, the spatial resolution in optical microscopy has increased to unprecedented levels by exploiting the fluorescence properties of the probe. At about the same time, Raman imaging techniques have emerged as a way to image inherent chemical information in a sample without using fluorescent probes. However, in many applications, the achievable resolution is limited to ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied Sciences
سال: 2020
ISSN: 2076-3417
DOI: 10.3390/app10124396