Efficient generation of transgenic pigs using equine infectious anaemia virus (EIAV) derived vector

Efficient generation of transgenic pigs using equine infectious anaemia virus (EIAV) derived vector.

Traditional methods of transgene delivery in livestock are inefficient. Recently, human immunodeficiency virus (HIV-1) based lentiviral vectors have been shown to offer an efficient transgene delivery system. We now extend this method by demonstrating efficient generation of transgenic pigs using an equine infectious anaemia virus derived vector. We used this vector to deliver a green fluoresce...

Characterisation of Equine Infectious Anaemia Virus (EIAV) strains

Serological method using recombinant S2 protein to differentiate equine infectious anemia virus (EIAV)-infected and EIAV-vaccinated horses.

We recently reported a highly protective attenuated live virus vaccine for equine infectious anemia virus (EIAV) based on a proviral construct (EIAVUKDeltaS2) with a genetically engineered mutation in the viral S2 gene that eliminates expression of this accessory protein. While the EIAVUKDeltaS2 vaccine provides protection from detectable infection by experimental challenge with highly virulent...

Infection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strain

The Chinese attenuated equine infectious anemia virus (EIAV) vaccine has successfully protected millions of equine animals from EIA disease in China. Given that the induction of immune protection results from the interactions between viruses and hosts, a better understanding of the characteristics of vaccine strain infection and host responses would be useful for elucidating the mechanism of th...

Equine Infectious Anaemia

Equine infectious anaemia (EIA) is a persistent viral infection of equids. The causative agent, EIA virus (EIAV) is a lentivirus in the family Retroviridae, subfamily Orthoretrovirinae. Other members of the genus Lentivirus include: bovine immunodeficiency virus; caprine arthritis encephalitis virus; feline immunodeficiency virus; human immunodeficiency virus 1; human immunodeficiency virus 2; ...