Efficient Biosynthesis of (S)-1-chloro-2-heptanol Catalyzed by a Newly Isolated Fungi Curvularia hominis B-36

(S)-1-chloro-2-heptanol is an enantiopure chemical of great value that can synthesize Treprostinil for treating primary pulmonary hypertension. In this work, a new strain B-36, capable asymmetric reduction 1-chloro-2-heptanone to (S)-1-chloro-2-heptanol, was screened and identified as Curvularia hominis B-36 (CCTCC M 2017654) based on the morphological internally transcribed spacer (ITS) sequence. The reductive capacity investigated whole-cell biocatalyst in bioreduction, excellent yield (97.2%) enantiomeric excess (ee) (99.9%) were achieved under optimal conditions follows: 75 mM 1-chloro-2-heptanone, K2HPO4-KH2PO4 (100 mM, pH 6.0), 50 g L−1 resting cells (dry cell weight; DCW), 15% (v/v) isopropanol co-substrate, 200 rpm, 30 °C, 20 h. scaled-up biocatalytic process accomplished at bioreactor 1.5 L working volume, showing superb (~97%) selectivity (99.9%). product purified characterized by NMR. novel catalyst synthesis route benign eco-friendly.