Efficient and rapid system of plant regeneration via protoplast cultures of Fagopyrum esculentum Moench

Abstract In the present study, a high yield of isolated protoplasts from agronomically important crop Fagopyrum esculentum was obtained by applying mixture cellulase, pectolyase, and driselase. We demonstrated that morphogenic callus-derived 1 × 10 6 per g fresh tissue. For hypocotyls used as protoplast source, number released cells twice lower. The protoplasts, embedded in an agarose matrix cultured modified Kao Michayluk media supplemented with phytosulfokine, re-enter cell cycle start to develop, forming microcalli. plating efficiency about 20% case hypocotyl- protoplasts. plant regeneration, medium different combinations cytokinin. Somatic embryogenesis organogenesis occur during cultivation protoplast-derived tissues, depending on applied source. first time, effective protoplast-to-plant system for F. has been developed.