Effects of warming procedures on the survivability of in vitro matured buffalo (Bubalus bubalis) oocytes vitrified by Cryotop
نویسندگان
چکیده
منابع مشابه
Effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (Bubalus bubalis) oocytes in vitro
AIM The aim was to assess the effects of fetal bovine serum (FBS) and estrus buffalo serum (EBS) on in vitro maturation rate of oocytes in buffalo. MATERIALS AND METHODS Maturation rate of oocytes was assessed in two maturation media supplemented with 20% FBS and EBS. Oocytes maturation rate was evaluated on the basis of cumulus cell expansion and extrusion of polar body after 24 h of in vitr...
متن کامل: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
Effects of cysteamine during in vitro maturation on viability and meiotic competence of vitrified buffalo oocytes
The aims of the present study were to assess the effects of cysteamine as an anti-oxidant on the rate of in vitro maturation (IVM) of buffalo oocytes (experiment 1), and their viability and nuclear status following vitrification (experiment 2). Immature oocytes with compact cumulus cells obtained from the ovaries of slaughtered animals were harvested and then cultured in the maturation medium w...
متن کاملFull-term Development of in vitro Produced-Vitrified Water Buffalo (Bubalus bubalis Linn) Embryos
Assisted reproductive technologies such as artificial insemination (AI) and multiple ovulation and embryo transfer (MOET) have been introduced to overcome reproductive inefficiencies and accelerate genetic gain. However, MOET in water buffalo is compromised by the poor ovulatory response of this animal species to hormonal treatment (Karaivanov 1986; Karaivanov et al. 1990; Misra et al. 1990; Cr...
متن کاملP-103: Effects of In Vitro Selenium Supplementation on Buffalo (Bubalus bubalis) Sperm Quality following Cryopreservation
Background: Artificial insemination is a valuable tool in genetic improvement programs and widely used breeding technique in farm animals. Semen processing and cryopreservation causes considerable damage to sperm DNA, motility apparatus, plasma membrane, acrosomal cap and thus, reduced fertility. Selenium is an integral part of glutathione peroxidase (GSH-Px), an enzyme which protects cell inte...
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ژورنال
عنوان ژورنال: Italian Journal of Animal Science
سال: 2007
ISSN: 1828-051X
DOI: 10.4081/ijas.2007.s2.735