Effects of pyriproxyfen and Bacillus thuringiensis Berliner, 1915 on enzymatic antioxidant defense system and hemocytes of Galleria mellonella (L., 1758) (Lepidoptera: Pyralidae)

With the increasing uses of biological control methods, knowing physiological and immunological effects these insecticides on insects is essential for them to be used safely in agricultural areas. The aim study determine pyriproxyfen Bacillus thuringiensis subsp. kurstaki individually as a mixture malondialdehyde levels (MDA), glutathione-s-transferase, acetylcholinesterase, cytochrome P450 enzyme activities hemolymph, midgut, fat body total (THC) differential hemocyte counts (DHC) fifth instar larvae Galleria mellonella (L., 1758) (Lepidoptera: Pyralidae) 24, 48 72 h after exposure under laboratory conditions (30 ± 1ºC, 65 5% RH). was conducted Animal Physiology Research Laboratory Department Biology, Faculty Science Letter, University Çukurova between 2016-2018. Effects antioxidant, detoxification MDA were changed depends time differences tissues. THC decreased 24 h, whereas it had increased h. DHC induced applied insecticide. This revealed that B. applications caused biochemical, reactions effected immune defense system by alterations counts.