Effects of propylene glycol on in vitro ruminal fermentation, methanogenesis, and microbial community structure

We evaluated the effects of propylene glycol (PG) on in vitro ruminal fermentation, methanogenesis, and microbial community structure. A completely randomized design was conducted incubation, 4 culture PG dose levels (0, 7.5, 15, 22.5 μL/g dry matter) were used trial. Based fermentation results, control group (0 matter, CON) second treatment (15.0 TRT) chosen for further analysis to explore bacterial archaeal The concentrations propanol, propanal, succinate increased linearly, whereas concentration l-lactate decreased linearly as doses increased. molar proportion propionate demonstrated a linear increase with increasing doses. In contrast propionate, acetate butyrate, acetate-to-propionate ratio addition markedly methane production without negative nutrient degradability. level, relative abundance Methanobrevibacter tended decrease, but that Methanomassiliicoccus significantly TRT group. At Bacteroidetes Prevotella numerically higher than CON Negativicutes class showed Succiniclasticum increase, Selenomonas decrease These results might be an inhibitor mitigate emission. However, small will limit application practices. Further research is needed determine whether use together other inhibitors may improve utilization reducing equivalents ([H]) production.