Effects of porcine epidemic diarrhea virus infection on Toll-like receptor expression and cytokine levels in porcine intestinal epithelial cells

To explore the role of Toll-like receptors (TLRs) and interferon (IFN) in innate immunity against porcine epidemic diarrhea virus (PEDV), we detected expression TLR genes PEDV-infected IPEC-J2 cells by real-time PCR. We also level α (IFN-α) γ (IFN-γ) enzyme-linked immunosorbent assay (ELISA). Results showed that exhibited a clear pathological change after PEDV infection at 24 h. In addition, TLR7, TLR9 TLR10 expressions were significantly upregulated Interestingly, patterns TLR2 TLR4 consistent different stages infection. The TLR3 decreased with increase time, but levels TLR5 TLR8 6 h 12 lower than those control group (p⟨0.01). There significant correlations among (p⟨0.05). Cytokine detection secretion IFN-α was higher (p⟨0.01), IFN-γ Therefore, our results suggest can induce immune responses intestinal jejunum epithelial cells, leading to changes receptors, regulate resistance affecting release downstream cytokines.