Effects of Particulate Matter 10 Inhalation on Lung Tissue RNA expression in a Murine Model

Background: Particulate matter 10 (PM10; airborne particles <10 ?m) inhalation has been demonstrated to induce airway and lung diseases. In this study, we investigate the effects of PM10 on RNA expression in tissues using a murine model. Methods: Female BALB/c mice were affected with PM10, ovalbumin (OVA), or both OVA PM10. was administered intranasally while intraperitoneally injected administered. Treatments occurred 4 times over 2-week period. Two days after final challenges, sacrificed. Full sequencing homogenates conducted. Results: While did not cell proliferation bronchoalveolar fluid lead hyper-responsiveness, it cause inflammation fibrosis. Levels interleukin 1?, tumor necrosis factor-?, transforming growth factor-? significantly elevated PM10-treated group, compared control group. The group also showed increased Rn45a , Snord22 Atp6v0c-ps2 Snora28 Snord15b Snora70 Mmp12 . Generally, genes associated splicing, DNA repair, inflammatory response, immune death, apoptotic processes highly expressed OVA/PM10 treatment produce greater than alone. However, OVA/PM10-treated show Clca1, Snord22, Retnla, Prg2, Tff2, Atp6v0c-ps2, Fcgbp when groups. These are response. Conclusion: Inhalation extensively altered inducing cellular inflammation, fibrosis, cytokines mouse model.