Effect of Unsaturated Fatty Acid Ratio In Vitro on Rumen Fermentation, Methane Concentration, and Microbial Profile

It is well known that dairy cows are fed diets with high fat content, which can adversely affect rumen fermentation. However, whether the effects of content on fermentation related to composition fatty acids (FA) for further study. We explored unsaturated acid (UFA) ratios in vitro rumen, methane concentration and microbial under same levels. The experiment included a low-unsaturated group (LU, UFA proportion: 42.8%), medium-unsaturated (MU, 56.9%), high-unsaturated (HU, 70.9%). incubation fluid pH NH3-N levels were not significantly different three groups. Total volatile (TVFA), acetate, propionate, butyrate, valerate MU had decreased trend compared LU (0.05 < p 0.1), no difference was found other (VFAs) among Furthermore, gas production kinetic parameters groups did differ significantly. group’s CH4 higher than HU (p 0.05). CO2 also Additionally, 16S rRNA sequencing results showed Shannon diversity value increased 0.05) group. Other alpha indices (Chao 1, observed species, ACE) proportion relative abundance Succinivibrionaceae_UCG_001 Fibrobacter Meanwhile, multiple Lachnospiraceae bacteria Overall, our findings indicated community system could be affected by elevating proportions UFA, affecting yield VFA, whereas reduced.