Effect of Trehalose Supplementation to Semen Extender on Quality of Cryopreserved Stallion Semen
نویسندگان
چکیده
The aim of the present study was to evaluate cryoprotective effect trehalose on stallion semen quality like progressive sperm motility, viability, acrosome integrity, membrane integrity. A total 42 ejaculates from six Marwari (seven ejaculate each) were used. Immediately after gross evaluation, filtered into a pre warm, graduated measuring bottle get gel free and it diluted with primary extender pellets. Secondary added divided three equal aliquots served as control, treatment 1(50 mM treahalose) 2 (150mM trehalose). At post-thaw stage there reduction in per cent integrity all groups. In T1 group motility viability higher (P<0.05) than control. However, control percentage hypo-osmotic swollen spermatozoa lower T1 and T2. intact deleterious T2 significant. conclusion, addition 50 improved post-thawing quality.
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ژورنال
عنوان ژورنال: International Journal of Current Microbiology and Applied Sciences
سال: 2021
ISSN: ['2319-7692', '2319-7706']
DOI: https://doi.org/10.20546/ijcmas.2021.1001.160