Effect of thrombin on the radioactive nucleotides of human washed platelets
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چکیده
منابع مشابه
Effect of thrombin on the radioactive nucleotides of human washed platelets.
Radioactive ATP and ADP were found in platelets after incubation of human platelet-rich plasma with either [8-(14)C]adenosine or [8-(14)C]ADP. Treatment of the labelled and washed platelets with thrombin indicated that, though considerable amounts of ATP and ADP were released to the supernatant, radioactive ATP and ADP remained predominantly in the cellular fraction. Breakdown of radioactive AT...
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In rabbit platelets, the metabolically active ATP pool equilibrates with the releasable ATP pool within 1 day. The studies showing this have now been extended to human platelets. Human platelets labeled with 14C-adenosine or 14C-adenine were incubated for up to 10 hr in vitro at 37 degrees C. After 10 hr, about 12% of the total platelet 14C-ATP and 14C-ADP had become releasable with thrombin (4...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1967
ISSN: 0306-3283
DOI: 10.1042/bj1050857