Effect of Lytic Enzymes of Acanthamoeba castellanii on Bacterial Cell Walls

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Effect of lytic enzymes of Acanthamoeba castellanii on bacterial cell walls.

Extracts of Acanthamoeba castellanii (Neff) contain alpha- and beta-glucosidase, beta-galactosidase, beta-N-acetylglucosaminidase, amylase, and peptidase. All of these activities are optimal between pH 3 and 4. These extracts also were found to clarify suspensions of cell walls from nine different gram-positive bacteria, including Micrococcus lysodeikticus. The pH optimum for the lytic activity...

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The lipids of Acanthamoeba castellanii (Neff) consist of 52% neutral lipids and 48% polar lipids. Triglycerides account for 75% and free sterols for 17% of the neutral lipids. The major phospholipids are phosphatidylcholine (45%), phosphatidylethanolamine (33%), phosphatidylserine (10%), a phosphoinositide (6%), and diphosphatidylglycerol (4%). The phosphoinositide is unique in that it contains...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1969

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.98.1.182-189.1969